DNA Primer Utilities

Javascript implementation of basic DNA sequence analyses.
© Simon Twigger, 1998
Basic Instructions:
  1. Enter your primer sequence (5'- 3') into the 'DNA Coding Sequence' box
  2. Press the Display... button to view the complement, reverse complement, size and melting temperature
  3. Show Restriction sites opens a fresh window displaying known restriction sites and their positions
  4. Translate opens a fresh window with a 3 frame translation of the DNA sequence.

This is still a work in progress, please check the Notes list at the foot of the page for known limitations and features yet to be implemented. Please send any suggestions to: Simon Twigger (simont@mcw.edu)
DNA Sequence (5' - 3' Coding Strand) Max length: 255bp
 
 
 
Complement (3' - 5' Non-coding)
Reverse Complement (5' - 3' Non-coding strand)
Tm (°C)
Length (bp)
Molecular Weight (Da)
 
Notes:
  • Tm (°C) = 81.5 + 16.6 x log([Na+]) + 41(#G + #C)/length - 500/length
    The [Na+] is taken as 0.1M. This formula is taken from the Operon data sheet.
  • Only copes with 4 DNA bases plus U: A,C,G,T,U. Add support for degenerate symbols?
  • Cater for different primer orientations (5'-3'/3'-5', coding/non-coding)