Summary: With complete sequences for the genomes of human and many other species now available, much of the attention in molecular biological research is rapidly turning to the characterization of organism-wide collections of gene products, often referred to as functional genomics or proteomics. Just as the human genome project catalyzed generational advances in DNA sequencing technology, current trends demand improved methods for efficient cloning, production and physical characterization of recombinant proteins. This course will focus on the practical aspects protein production and characterization, including the steps most commonly encountered in the development of targets for three-dimensional structural characterization by NMR or x-ray crystallography. New methods and optimization of standard approaches for high-throughput applications will be emphasized, including techniques ranging from chromatography and electrophoresis to mass spectrometery, fluorescence and NMR spectroscopy.
Meetings: Mondays and Wednesdays, 9:00-10:30, Biochemistry library (BSB 373)
Required Text: David Sheehan, Physical Biochemistry: Principles and Applications
Grades: Based in equal parts upon results of two exams and final in-class literature presentation.
Lecturers and contact information
Syllabus and lecture notes - authorized access only!
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