Date

Lecturer

Topic

Mon 8/25

Volkman

Overview; expression and translation PPT(3.7MB)

Wed 8/27

Volkman

Protein folding, structure and function PPT(5.6MB)

Mon 9/1

Labor Day

Production

Wed 9/3

Peterson

Bacterial protein expression
PPT(1.6MB) pdf1 pdf2 pdf3 pdf4 doc5

Mon 9/8

Dahms

Eukaryotic protein expression PPT(14.6MB)

Wed 9/10

Wakim

Solid-phase synthesis; chemical modification PPT(15.1MB)

Mon 9/15

Vinarov

Cell-free protein expression in structural genomics:
Dr. Dmitriy Vinarov, Center for Eukaryotic Structural Genomics, UW-Madison

Identification and Purification

Wed 9/17

Wakim

Mass spectrometry; post-translational modifications PPT(31.7MB)

Mon 9/22

Haas

SDS-PAGE, Western blot

Wed 9/24

Haas

2D electrophoresis with MS detection

Mon 9/29

Haas

Chromatography - ion exchange, size exclusion

Wed 10/1

Haas

Affinity chromatography

Mon 10/6

Wakim

Reverse phase HPLC; Amino acid analysis PPT48MB

Wed 10/8

Wakim

Disulfide mapping; protein sequencing PPT14MB

Mon 10/13

Exam 1: protein production, identification and purification

Characterization: folding, aggregation state, and structure

Wed 10/15

Volkman

Analytical ultracentrifugation PPT3.4MB

Mon 10/20

Twining

UV-vis and fluorescence spectroscopy PPT12.6MB

Wed 10/22

Volkman

NMR spectroscopy - PFG diffusion

Mon 10/27

Volkman

NMR and circular dichroism analysis of folding PPT9.2MB

Characterization: intermolecular interactions

Wed 10/29

no class

Mon 11/3

Volkman

NMR binding: shift mapping, H/D exchange and NOE sat. transfer PPT2.3MB

Wed 11/5

Dolmer

Calorimetry; Dr. Klavs Dolmer, Univ. Ill-Chicago PPT5MB

Mon 11/10

Twining

Fluorescence polarization and anisotropy; FRET PPT4.3MB

Wed 11/12

Volkman

Surface plasmon resonance PPT2.2MB EXCEL SHEET

Mon 11/17

Barbieri

GST pull-down; Co-immunoprecipitation

Wed 11/19

Barbieri

ELISA; Scatchard analysis

Mon 11/24

Exam 2: protein characterization

Genomics and proteomics

Wed 11/26
10:30-noon

Barbieri

Sequence analysis algorithms-note special time

Mon 12/1

Barbieri

Protein evolution and homology

Wed 12/3

Volkman

Structural genomics

Tues 12/9

Sem

Chemical proteomics; Dr. Daniel Sem, Dept. of Chemistry, Marquette Univ. note special day

Wed 12/10

Students

Paper presentations

Mon 12/15

Students

Paper presentations

Wed 12/17

Students

Paper presentations