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Sample Clean up Using Zip-Tips in Preparation for MALDI-TOF Mass Analysis:

Zip-Tips are pipette tips that contain immobilized C18, C4 or some other resin attached at their very tip occupying about 0.5µl volume. For most purposes we use C18 resin. Our usual protocol is:

  1. Use a P20 pipetter set to 10µl for Zip-Tips
  2. Wash the Zip-Tip with 0.1% trifluroacetic acid (TFA) in acetonitrile
  3. Wash the Zip-Tip with 0.1% TFA in 1:1 acetonitrile:water
  4. quilibrate the Zip-Tip twice with 0.1% TFA in water
  5. The sample, typically dissolved in 10 µl of 0.1% TFA, is passed through the Zip-Tips repeatedly by pipeting in and out to bind the sample to the resin.
  6. Wash the Zip-Tip three times with 0.1% TFA, 5% methanol in water
  7. Elute the sample from the Zip-Tip in 1.8µl of matrix, typically alpha-cyano-4-hydroxycinnamic acid in 0.1% TFA 50% acetonitrile, directly on the MALDI-TOF sample plate.
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